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Department of Physics and Astronomy
University of Mississippi

Events

Event Information:

  • Tue
    27
    Oct
    2020

    Colloquium: Holography and the Pandemic — Using Holographic Video Microscopy to Detect Viruses and Antibodies

    4:00 pmZoom Meeting. See Below.

    David G. Grier
    Department of Physics and the Center for Soft Matter Research
    New York University

    Holography and the Pandemic: Using Holographic Video Microscopy to Detect Viruses and Antibodies

    The hologram of a microscopic object encodes information about that object's size, shape, composition and three-dimensional position. Often, that information is retrieved by computing a three-dimensional reconstruction of the complex medium and then analyzing the result. The three-dimensional reconstruction, however, contains no more information than the original two-dimensional hologram (and usually less). In special cases, the recorded hologram instead can be fit, pixel-by-pixel, to the exact Lorenz-Mie theory of light scattering. For a micrometer-scale colloidal sphere, this analysis yields the position to within a few nanometers over a range extending to hundreds of micrometers. More importantly, it yields the sphere's diameter to within a couple of nanometers. This is fine enough to monitor molecules and viruses binding to the surfaces of functionalized beads simply by watching the beads grow larger in real time. The same analysis yields the bead's refractive index with part-per-thousand resolution, which elegantly solves the barcoding problem for multiplexed binding assays. This talk will explain how to use holographic microscopy for precision particle characterization. It then will showcase a few practical and scientific applications that illustrate the power of the technique before diving into the emergency application for COVID-19

     

     

     

    Join Zoom Meeting
    https://olemiss.zoom.us/j/213798950

    Meeting ID: 213 798 950